Abstract
The Escherichia coli nucleoid contains DNA in a condensed but functional form. Analysis of proteins released from isolated spermidine nucleoids after treatment with DNase I reveals significant amounts of two proteins not previously detected in wild-type E. coli. Partial amino-terminal sequencing has identified them as the products of rdgC and yejK. These proteins are strongly conserved in gram-negative bacteria, suggesting that they have important cellular roles.
MeSH terms
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Amino Acid Sequence
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Bacterial Proteins / chemistry*
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Bacterial Proteins / genetics
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Bacterial Proteins / isolation & purification
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Conserved Sequence
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Deoxyribonuclease I
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Escherichia coli / chemistry*
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Escherichia coli / genetics
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Escherichia coli / metabolism
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Escherichia coli Proteins*
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Gram-Negative Bacteria / chemistry
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Molecular Sequence Data
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Organelles / chemistry*
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Spermidine / metabolism*
Substances
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Bacterial Proteins
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Escherichia coli Proteins
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RdgC protein, E coli
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YejK protein, E coli
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Deoxyribonuclease I
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Spermidine