Abstract
We have investigated the intracellular roles of an Xklp2-related kinesin motor, KRP(180), in positioning spindle poles during early sea urchin embryonic cell division using quantitative, real-time analysis. Immunolocalization reveals that KRP(180) concentrates on microtubules in the central spindle, but is absent from centrosomes. Microinjection of inhibitory antibodies and dominant negative constructs suggest that KRP(180) is not required for the initial separation of spindle poles, but instead functions to transiently position spindle poles specifically during prometaphase.
Publication types
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
Amino Acid Sequence
-
Animals
-
Calcium-Binding Proteins / genetics
-
Calcium-Binding Proteins / isolation & purification*
-
Cell Cycle Proteins / genetics
-
Dimerization
-
Embryo, Nonmammalian / ultrastructure*
-
Fluorescent Antibody Technique
-
Kinesins / genetics
-
Metaphase*
-
Microtubule-Associated Proteins / genetics
-
Microtubule-Associated Proteins / isolation & purification
-
Models, Biological
-
Molecular Motor Proteins*
-
Molecular Sequence Data
-
Muscle Proteins / genetics
-
Muscle Proteins / isolation & purification*
-
Sea Urchins
-
Sequence Homology, Amino Acid
-
Spindle Apparatus / ultrastructure*
-
Xenopus Proteins*
Substances
-
Calcium-Binding Proteins
-
Cell Cycle Proteins
-
KIF15 protein, Xenopus
-
Microtubule-Associated Proteins
-
Molecular Motor Proteins
-
Muscle Proteins
-
Xenopus Proteins
-
kinesin-II
-
Kinesins