A rod conformation of the Pyrococcus furiosus Rad50 coiled coil

Proteins. 2021 Feb;89(2):251-255. doi: 10.1002/prot.26005. Epub 2020 Sep 25.

Abstract

The Rad50-Mre11 nuclease complex plays a vital role in DNA repair in all domains of life. It recognizes and processes DNA double-strand breaks. Rad50 proteins fold into an extended structure with a 20 to 60 nm long coiled coil connecting a globular ABC ATPase domain with a zinc hook dimerization domain. A published structure of an archaeal Rad50 zinc hook shows coiled coils pointing away from each other. Here we present the crystal structure of an alternate conformation displaying co-aligned coiled coils. Archaeal Rad50 may thus switch between rod-shaped and ring-like conformations as recently proposed for a bacterial homolog.

Keywords: DNA repair; Mre11; Rad50; SMC; SMC-like; coiled coil; rod; zinc hook.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Motifs
  • Archaeal Proteins / chemistry*
  • Archaeal Proteins / genetics
  • Archaeal Proteins / metabolism
  • Binding Sites
  • Cations, Divalent
  • Cloning, Molecular
  • Crystallography, X-Ray
  • DNA Repair*
  • DNA, Archaeal / chemistry*
  • DNA, Archaeal / genetics
  • DNA, Archaeal / metabolism
  • Endodeoxyribonucleases / chemistry*
  • Endodeoxyribonucleases / genetics
  • Endodeoxyribonucleases / metabolism
  • Escherichia coli / genetics
  • Escherichia coli / metabolism
  • Exodeoxyribonucleases / chemistry*
  • Exodeoxyribonucleases / genetics
  • Exodeoxyribonucleases / metabolism
  • Gene Expression
  • Genetic Vectors / chemistry
  • Genetic Vectors / metabolism
  • Humans
  • Models, Molecular
  • Protein Binding
  • Protein Conformation, alpha-Helical
  • Protein Interaction Domains and Motifs
  • Protein Multimerization
  • Pyrococcus furiosus / genetics*
  • Pyrococcus furiosus / metabolism
  • Recombinant Proteins / chemistry
  • Recombinant Proteins / genetics
  • Recombinant Proteins / metabolism
  • Structural Homology, Protein
  • Zinc / chemistry*
  • Zinc / metabolism

Substances

  • Archaeal Proteins
  • Cations, Divalent
  • DNA, Archaeal
  • Recombinant Proteins
  • Endodeoxyribonucleases
  • Exodeoxyribonucleases
  • Mre11 protein, archaeal
  • Rad50 protein, archaeal
  • Zinc