Molecular recognition of chymotrypsin by the serine protease inhibitor ecotin from Yersinia pestis

J Biol Chem. 2011 Jul 8;286(27):24015-22. doi: 10.1074/jbc.M111.225730. Epub 2011 Apr 29.

Abstract

Resistance to antibiotics is a problem not only in terms of healthcare but also biodefense. Engineering of resistance into a human pathogen could create an untreatable biothreat pathogen. One such pathogen is Yersinia pestis, the causative agent of plague. Previously, we have used a bioinformatic approach to identify proteins that may be suitable targets for antimicrobial therapy and in particular for the treatment of plague. The serine protease inhibitor ecotin was identified as one such target. We have carried out mutational analyses in the closely related Yersinia pseudotuberculosis, validating that the ecotin gene is a virulence-associated gene in this bacterium. Y. pestis ecotin inhibits chymotrypsin. Here, we present the structure of ecotin in complex with chymotrypsin to 2.74 Å resolution. The structure features a biologically relevant tetramer whereby an ecotin dimer binds to two chymotrypsin molecules, similar to what was observed in related serine protease inhibitor structures. However, the vast majority of the interactions in the present structure are distinctive, indicating that the broad specificity of the inhibitor for these proteases is based largely on its capacity to recognize features unique to each of them. These findings will have implications for the development of small ecotin inhibitors for therapeutic use.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chymotrypsin / antagonists & inhibitors
  • Chymotrypsin / chemistry
  • Chymotrypsin / genetics
  • Chymotrypsin / metabolism
  • Crystallography, X-Ray
  • Humans
  • Periplasmic Proteins / chemistry*
  • Periplasmic Proteins / genetics
  • Periplasmic Proteins / metabolism
  • Plague / genetics
  • Plague / metabolism
  • Protein Structure, Quaternary
  • Serine Proteinase Inhibitors / chemistry*
  • Serine Proteinase Inhibitors / genetics
  • Serine Proteinase Inhibitors / metabolism
  • Virulence Factors / chemistry*
  • Virulence Factors / genetics
  • Virulence Factors / metabolism
  • Yersinia pestis / chemistry*
  • Yersinia pestis / genetics
  • Yersinia pestis / metabolism
  • Yersinia pestis / pathogenicity
  • Yersinia pseudotuberculosis / chemistry
  • Yersinia pseudotuberculosis / genetics
  • Yersinia pseudotuberculosis / metabolism
  • Yersinia pseudotuberculosis / pathogenicity

Substances

  • Periplasmic Proteins
  • Serine Proteinase Inhibitors
  • Virulence Factors
  • ecotin protein, Yersinia pestis
  • Chymotrypsin

Associated data

  • PDB/2Y6T