Analysis of CGR8 embryoid bodies differentiating for 3 or 10 days. Culturing of most embryonic stem (ES) cells require embryonic fibroblast (MEF) feeder cells which may affect their gene expression profiles. CGR8 cells are MEF-independent and represent a powerful model of ES cell differentiation.
Gissel C, Voolstra C, Doss MX, Koehler CI et al. An optimized embryonic stem cell model for consistent gene expression and developmental studies: a fundamental study. Thromb Haemost 2005 Oct;94(4):719-27. PMID: 16270623
