Coffee Silverskin and Spent Coffee Suitable as Neuroprotectors against Cell Death by Beauvericin and α-Zearalenol: Evaluating Strategies of Treatment

Toxins (Basel). 2021 Feb 10;13(2):132. doi: 10.3390/toxins13020132.

Abstract

Coffee silverskin and spent coffee have been evaluated in a neuroblastoma cell line (SH-SY5Y cells) against beauvericin (BEA) and α-zearalenol (α-ZEL)-induced cytotoxicity with different strategies of treatment. First, the direct treatment of mycotoxins and coffee by-products extracts in SH-SY5Y cells was assayed. IC50 values for α-ZEL were 20.8 and 14.0 µM for 48 h and 72 h, respectively and, for BEA only at 72 h, it was 2.5 µM. Afterwards, the pre-treatment with spent coffee obtained by boiling water increased cell viability for α-ZEL at 24 h and 48 h from 10% to 16% and from 25% to 30%, respectively; while with silverskin coffee, a decrease was observed. Opposite effects were observed for BEA where an increase for silverskin coffee was observed at 24 h and 48 h, from 14% to 23% and from 25% to 44%, respectively; however, a decrease below 50% was observed for spent coffee. Finally, the simultaneous treatment strategy for the highest concentration assayed in SH-SY5Y cells provided higher cytoprotection for α-ZEL (from 44% to 56% for 24 h and 48 h, respectively) than BEA (30% for 24 h and 48 h). Considering the high viability of coffee silverskin extracts for SH-SY5Y cells, there is a forthcoming promising use of these unexploited residues in the near future against mycotoxins effects.

Keywords: SH-SY5Y cells; beauvericin; coffee silverskin; spent coffee; α-zearalenol.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Death / drug effects*
  • Cell Line, Tumor
  • Coffee* / chemistry
  • Cytoprotection
  • Depsipeptides / toxicity*
  • Dose-Response Relationship, Drug
  • Humans
  • Inhibitory Concentration 50
  • Neurons / drug effects*
  • Neurons / pathology
  • Neuroprotective Agents / isolation & purification
  • Neuroprotective Agents / pharmacology*
  • Plant Extracts / isolation & purification
  • Plant Extracts / pharmacology*
  • Seeds* / chemistry
  • Time Factors
  • Zeranol / analogs & derivatives*
  • Zeranol / toxicity

Substances

  • Coffee
  • Depsipeptides
  • Neuroprotective Agents
  • Plant Extracts
  • zearalenol
  • beauvericin
  • Zeranol