Abstract
Sequence analysis showed that the cyd operon is immediately upstream of the tol-pal region. Northern (RNA) blot analysis demonstrated that the transcript for the cyd operon terminates just before the promoter for transcription of the tol genes. The cyd transcript contains cydA cydB followed by two open reading frames: orfC, encoding a 37-residue peptide, and orfD, encoding a 97-residue peptide. Both OrfC and OrfD are synthesized in minicells.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Bacterial Proteins / biosynthesis
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Bacterial Proteins / chemistry
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Bacterial Proteins / genetics*
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Base Sequence
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Cytochrome b Group
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Cytochromes / genetics*
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Electron Transport Chain Complex Proteins*
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Escherichia coli / genetics*
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Escherichia coli Proteins*
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Molecular Sequence Data
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Open Reading Frames / genetics
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Operon*
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Oxidoreductases / genetics*
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RNA, Bacterial / genetics
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RNA, Messenger / genetics
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Trans-Activators*
Substances
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Bacterial Proteins
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Cytochrome b Group
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Cytochromes
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Electron Transport Chain Complex Proteins
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Escherichia coli Proteins
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RNA, Bacterial
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RNA, Messenger
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Trans-Activators
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mraZ protein, E coli
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fhlA protein, E coli
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Oxidoreductases
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cytochrome bd terminal oxidase complex, E coli