F0F1-ATPase genes from an archaebacterium, Methanosarcina barkeri

Biochem Biophys Res Commun. 1997 Dec 18;241(2):427-33. doi: 10.1006/bbrc.1997.7809.

Abstract

It has been known that an archaebacterium Methanosarcina barkeri strain MS (DSM 800) has a V-type ATPase (Inatomi, K., et al. (1989) J. Biol. Chem. 264, 10954-10959). Here, we report cloning of a cluster of F0F1-ATPase genes from the same organism, the first ever found in archaebacteria. The cluster and encoded subunits exhibit several unusual features such that a gene for delta subunit is lacking, F0-b subunit is unusually large, and gamma subunit is split into two peptide fragments. Attempts to detect F0F1-ATPase proteins and mRNA have been unsuccessful and therefore it is not certain if this gene cluster is really expressed in the cell.

Publication types

  • Comparative Study

MeSH terms

  • Amino Acid Sequence
  • Base Composition
  • Genes, Archaeal*
  • Methanosarcina barkeri / enzymology
  • Methanosarcina barkeri / genetics*
  • Molecular Sequence Data
  • Multigene Family*
  • Polymerase Chain Reaction
  • Protein Conformation
  • Proton-Translocating ATPases / genetics*
  • Restriction Mapping
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid

Substances

  • Proton-Translocating ATPases

Associated data

  • GENBANK/AF028006