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    nrdB ribonucleoside-diphosphate reductase 1 subunit beta [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 946732, updated on 4-Feb-2024

    Summary

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    Gene symbol
    nrdB
    Gene description
    ribonucleoside-diphosphate reductase 1 subunit beta
    Primary source
    ASAP:ABE-0007386
    Locus tag
    b2235
    See related
    ECOCYC:EG10661
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK2227; ftsB
    Summary
    Reducing equivalents provided by glutaredoxin or thioredoxin. [More information is available at EcoGene: EG10661]. The B2 protein of ribonucleoside-diphosphate reductase contains the tyrosyl radical-dinuclear iron center, which is thought to initiate catalysis by long-range electron transfer. [More information is available at EcoCyc: EG10661].
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    Genomic context

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    Sequence:
    NC_000913.3 (2347384..2348514)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene protein YpaB Neighboring gene ribonucleoside-diphosphate reductase 1 subunit alpha Neighboring gene protein YoaM Neighboring gene ferredoxin-like diferric-tyrosyl radical cofactor maintenance protein YfaE Neighboring gene putative lipopolysaccharide kinase InaA Neighboring gene pseudo

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Redox-linked structural changes in ribonucleotide reductase. Offenbacher AR, et al. J Am Chem Soc, 2009 Jun 10. PMID 19489635, Free PMC Article
    2. Cation mediation of radical transfer between Trp48 and Tyr356 during O2 activation by protein R2 of Escherichia coli ribonucleotide reductase: relevance to R1-R2 radical transfer in nucleotide reduction? Saleh L, et al. Biochemistry, 2006 Jul 25. PMID 16846225
    3. Structure of Escherichia coli ribonucleotide reductase R2 in space group P6122. Sommerhalter M, et al. Acta Crystallogr D Biol Crystallogr, 2005 Dec. PMID 16301799
    4. Local and global effects of metal binding within the small subunit of ribonucleotide reductase. Pierce BS, et al. J Am Chem Soc, 2005 Mar 16. PMID 15755183
    5. Characterization of NO adducts of the diiron center in protein R2 of Escherichia coli ribonucleotide reductase and site-directed variants; implications for the O2 activation mechanism. Lu S, et al. J Biol Inorg Chem, 2004 Oct. PMID 15311337

    See all (113) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Elevated Levels of the Escherichia coli nrdAB-Encoded Ribonucleotide Reductase Counteract the Toxicity Caused by an Increased Abundance of the beta Clamp.
      Title: Elevated Levels of the Escherichia coli nrdAB-Encoded Ribonucleotide Reductase Counteract the Toxicity Caused by an Increased Abundance of the β Clamp.
    2. Subunit Interaction Dynamics of Class Ia Ribonucleotide Reductases: In Search of a Robust Assay.
      Title: Subunit Interaction Dynamics of Class Ia Ribonucleotide Reductases: In Search of a Robust Assay.
    3. The present results identifying a large, new set of E. coli mutator strains with mutations of nrdAB ribonucleotide reductase(RNR)confirm the important control that RNR exerts on the cellular mutation rate and provide novel insights into the RNR feedback regulatory mechanisms.
      Title: Novel mutator mutants of E. coli nrdAB ribonucleotide reductase: insight into allosteric regulation and control of mutation rates.
    4. N-terminus verified by Edman degradation on mature peptide
      Title: Cloning, overproduction, and purification of the B2 subunit of ribonucleoside-diphosphate reductase.
    5. analysis of key residues in the dimerization process of ribonucleotide reductase protein R1
      Title: Nucleotide-dependent formation of catalytically competent dimers from engineered monomeric ribonucleotide reductase protein R1.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables identical protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables iron ion binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables oxidoreductase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    contributes_to ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in 2'-deoxyribonucleotide biosynthetic process IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within deoxyribonucleotide biosynthetic process IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in deoxyribonucleotide biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within nucleobase-containing small molecule interconversion IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in ribonucleoside diphosphate metabolic process IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in cytoplasm IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in cytosol HDA PubMed 
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of ribonucleoside-diphosphate reductase complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of ribonucleoside-diphosphate reductase complex IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    ribonucleoside-diphosphate reductase 1 subunit beta
    NP_416738.1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      2347384..2348514
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_416738.1 ribonucleoside-diphosphate reductase 1 subunit beta [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_416738.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A8T5YJX1
      Conserved Domains (1) summary
      PRK09101
      Location:1 → 376
      nrdB; ribonucleotide-diphosphate reductase subunit beta; Reviewed
    Nucleotide Protein
    Heading Accession and Version

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