Shotgun genome sequencing of Neofusicoccum parvum (isolate UCRNP2) - SRA

SRX220832: Shotgun genome sequencing of Neofusicoccum parvum (isolate UCRNP2)
1 ILLUMINA (Illumina HiSeq 2000) run: 37M spots, 7.4G bases, 4.8Gb downloads

Design: Genomic DNA was extracted mycelia growing on PDA plate. A single DNA library was prepared from 5 ug of total genomic DNA that was randomly fragmented using the Bioruptor sonicator (Diagenode). The library was prepared with the NEB Next DNA Sample Prep Master Mix according to manufacturer's instructions (New England Biolabs, MA USA). Fragments were purified using the Nucleotrap kit (Clontech) and eluted in 30 ul elution buffer and PCR-enriched using 14 PCR cycles. Paired end adapters and PCR primers were obtained from Illumina. Library quality and quantity were confirmed before sequencing using a Bioanalyzer (Agilent).

Submitted by: UC Davis

Study: Neofusicoccum parvum UCRNP2 isolate:UCRNP2 Genome sequencing and assembly

PRJNA187491 • SRP018315 • All experiments • All runs

show Abstracthide Abstract

Neofusicoccum parvum is an important canker pathogen of woody plants, including grapevine (Vitis vinifera), Eucalyptus, pome and stone fruit trees, blueberry, and avocado.

Sample: Neofusicoccum parvum isolate UCRNP2

SAMN01906718 • SRS388388 • All experiments • All runs

Organism: Neofusicoccum parvum UCRNP2

Library:

Instrument: Illumina HiSeq 2000

Strategy: WGS

Source: GENOMIC

Selection: unspecified

Layout: PAIRED

Spot descriptor:

1 forward101 reverse

Runs: 1 run, 37M spots, 7.4G bases, 4.8Gb

Run	# of Spots	# of Bases	Size	Published
SRR654031	36,999,281	7.4G	4.8Gb	2013-05-04

ID:: 308337

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Sequence Read Archive

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