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SRX18725156: Illumina WGS of Phasmarhabditis papillosa (Nematoda)
1 ILLUMINA (Illumina NovaSeq 6000) run: 30.5M spots, 9.2G bases, 2.9Gb downloads

Design: DNA was extracted in-house from multiple individuals (cultures) for nematode and tardigrade species, or from single specimens for all other taxa. For microfauna and mesofauna, the whole specimen was used for DNA extraction, while body parts of macrofauna were used. A non-destructive DNA extraction protocol was followed, according to Gilbert et al. (2007), except for Illumina batch 8 where the standard DNeasy Tissue Kit was used (Qiagen Inc.). Library preparations were performed in-house for earlier Illumina batches, and directly at Novogene from batch 7 onwards according to the NEBNext Ultra II DNA Library Prep Kit. Gilbert MTP, Moore W, Melchior L, Worobey M (2007) DNA Extraction from Dry Museum Beetles without Conferring External Morphological Damage. PLoS ONE 2(3): e272. doi:10.1371/journal.pone.0000501
Submitted by: LOEWE Centre for Translational Biodiversity Genomics
Study: Soil invertebrates from the LOEWE TBG and Senckenberg project 'Metagenomic monitoring of soil communities' (MetaInvert)
show Abstracthide Abstract
The MetaInvert database serves to improve species identification through shotgun metagenomics, to reveal evolutionary relationships, special adaptations, and host-microbiome associations. We are developing and adapting molecular approaches to study the structure, composition and functions of hard-to-observe organisms.Sequences were generated within Senckenberg and the LOEWE Centre for Translational Biodiversity Genomics (TBG) with funding from LOEWE in the MetaInvert project, and as part of the Central-European Soil Invertebrate Genome Initiative (SIGI). The vouchers are deposited in the collections of the Senckenberg Museum of Natural History Goerlitz (SMNG), Germany. The species were identified by the taxon experts of SMNG and ECT Oekotoxikologie GmbH, Florsheim, Germany.
Sample:
SAMN25040872 • SRS16166310 • All experiments • All runs
Library:
Name: b3_b5__P3_2
Instrument: Illumina NovaSeq 6000
Strategy: WGS
Source: GENOMIC
Selection: RANDOM
Layout: PAIRED
Runs: 1 run, 30.5M spots, 9.2G bases, 2.9Gb
Run# of Spots# of BasesSizePublished
SRR2276469430,517,5269.2G2.9Gb2023-11-16

ID:
25804752

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