Neomenia permagna transcriptome data - SRA

SRX23536360: Neomenia permagna transcriptome data
1 ILLUMINA (Illumina NovaSeq X) run: 24.4M spots, 4.9G bases, 2Gb downloads

Design: RNA was extracted as described by Kocot et al. (2019). RNA concentration was measured using a Qubit 3.0 (Thermo Fisher) fluorometer with the RNA High Sensitivity kit, RNA purity was assessed by measuring the 260/280 nm absorbance ratio using a Nanodrop Lite (Thermo Fisher), and RNA integrity was evaluated using a 1% SB agarose gel. cDNA synthesis was performed using 1 ng of total RNA using the Clontech SMART-Seq HT kit. An Illumina sequencing library was then prepared in house using the Nextera XT DNA Library Preparation Kit with 0.15 ng of cDNA. Final library quality was assessed using an Agilent Fragment Analyzer with the NGS 1-6000 bp kit and sent to Psomagen (Cambridge, MA) for sequencing on a NovaSeq S4 flowcell for paired-end sequencing

Submitted by: The University of Alabama

Study: Two draft genomes of enigmatic Solenogastres (Aplacophora, Mollusca) Epimenia babai and Neomenia megatrapezata

PRJNA1071799 • SRP488211 • All experiments • All runs

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Illumina and Oxford Nanopore genomic data from the solenogasters Epimenia babai and Neomenia megatrapeza

Sample: Neomenia permagna transcriptome data

SAMN39711723 • SRS20384890 • All experiments • All runs

Organism: Neomenia permagna

Library:

Name: Ap26.2C

Instrument: Illumina NovaSeq X

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: Oligo-dT

Layout: PAIRED

Runs: 1 run, 24.4M spots, 4.9G bases, 2Gb

Run	# of Spots	# of Bases	Size	Published
SRR27874471	24,395,224	4.9G	2Gb	2024-02-05

ID:: 31793758

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