UCE target enrichment of Caenolestes_caniventer_LSUMZ_25395: muscle/l... - SRA

SRX3200002: UCE target enrichment of Caenolestes_caniventer_LSUMZ_25395: muscle/liver tissue
1 ILLUMINA (Illumina HiSeq 3000) run: 4.3M spots, 1.3G bases, 544.7Mb downloads

Design: We prepared DNA libraries using the Kapa Biosystems Hyper Prep Kit for Illumina platforms with dual indexed iTru adapters (Glenn et al. 2016). We combined the resulting DNA libraries in equimolar pools of eight samples and enriched each pool for 5,060 UCE loci using the Tetrapods-UCE-5Kv1 probeset(Faircloth etal. 2012) sold byMYcroarray (Ann Arbor, MI). We determined the size distribution of enriched libraries with an Agilent Bioanalyzer and removed remaining adapter dimer from pools where it was present using a 0.8 bead cleanup. We then quantiÞed the enriched libraries with a Qubit 2.0 ßuorometer and pooled them in equimolar ratios.

Submitted by: Louisiana State University

Study: Phylogenetic relationships of mammals inferred using ultra conserved elements

PRJNA390442 • SRP118182 • All experiments • All runs

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Our goal is to improve resolution of the phylogenetic relationships among mammals.

Sample:

SAMN07542990 • SRS2527005 • All experiments • All runs

Organism: Caenolestes caniventer

Library:

Name: Caenolestes_caniventer_LSUMZ_25395

Instrument: Illumina HiSeq 3000

Strategy: WGS

Source: GENOMIC

Selection: Hybrid Selection

Layout: PAIRED

Runs: 1 run, 4.3M spots, 1.3G bases, 544.7Mb

Run	# of Spots	# of Bases	Size	Published
SRR6053056	4,266,319	1.3G	544.7Mb	2017-09-20

ID:: 4503746

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