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SRX2966574: DNA-Seq of Strongylocentrotus pallidus: gonad
1 ILLUMINA (Illumina HiSeq 2500) run: 74.9M spots, 15G bases, 8.4Gb downloads

Design: Gonad tissues were preserved in 95% EtOH and total DNA extracted. A 6 ng aliquot of genomic DNA from a single individual was fragmented using a BioRuptor (Diagenode Inc., Denville, NJ) using six cycles of 30s on/off at the ÒHighÓ setting for 5 min. Paired-end libraries were prepared from the fragmented genomic DNA with mean insert sizes of 200 Ð 300 bp.
Submitted by: University of California
Study: Strongylocentrotus and Mesocentrotus taxa raw sequence reads
show Abstracthide Abstract
Genetic differences that accumulate between species can result from the action of either random genetic drift or natural selection. However, the relative importance of drift vs. selection in causing the differences observed between species remains poorly understood. This study will used closely related species of Strongylocentrotid sea urchins to address several fundamental questions in the field of evolutionary genetics. First, what proportion of the genetic changes observed between species has been caused by natural selection? Second, have the selected changes occurred recently within existing species or do they reflect adaptation occurring further back in time? Third, what are the specific DNA regions that show evidence of past natural selection and can these changes be explained by known biological processes (such as reproduction or disease resistance)? The complete genome sequences from eight species of sea urchin were used to investigate these questions. The results provide new insights into the importance of selection as a force for genome evolution.
SAMN07269097 • SRS2323591 • All experiments • All runs
Name: VJCQB3KMK002
Instrument: Illumina HiSeq 2500
Strategy: WGS
Selection: RANDOM
Layout: PAIRED
Runs: 1 run, 74.9M spots, 15G bases, 8.4Gb
Run# of Spots# of BasesSizePublished


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