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SRX19844990: Low coverage genome shotgun sequencing of Cyclopes didactylus CycDid_MNHN1998-234
1 ILLUMINA (Illumina HiSeq 2500) run: 1.8M spots, 132M bases, 71.7Mb downloads

Design: Libary preparation folloed Meyer & Kircher (2010)
Submitted by: CNRS - Universite de Montpellier
Study: Raw sequence reads from low coverage shotgun genome sequencing of living xenarthrans
show Abstracthide Abstract
Xenarthra (armadillos, sloths, and anteaters) constitutes one of the four major clades of placental mammals. Despite their phylogenetic distinctiveness in mammals, a reference phylogeny is still lacking for the 31 described species. Here we used Illumina shotgun sequencing to assemble 33 new complete mitochondrial genomes, establishing Xenarthra as the first major placental clade to be fully sequenced at the species level for mitogenomes. The resulting data set allowed the reconstruction of a robust phylogenetic framework and timescale that are consistent with previous studies conducted at the genus level using nuclear genes. Incorporating the full species diversity of extant xenarthrans points to a number ofinconsistencies in xenarthran systematics and species definition. We propose to split armadillos into two distinct families Dasypodidae (dasypodines) and Chlamyphoridae (euphractines, chlamyphorines, and tolypeutines) to better reflect their ancient divergence, estimated around 42 Ma. Species delimitation within long-nosed armadillos (genus Dasypus) appeared more complex than anticipated, with the discovery of a divergent lineage in French Guiana. Diversification analyses showed Xenarthra to be an ancient clade with a constant diversification rate through time with a species turnover driven by high but constant extinction. We also detected a significant negative correlation between speciation rate and past temperature fluctuations with an increase in speciation rate corresponding to the general cooling observed during the last 15 My. Biogeographic reconstructions identified the tropical rainforest biome of Amazonia and the Guiana Shield as the cradle of xenarthran evolutionary history with subsequent dispersions into more open and dry habitats.
SAMN34027139 • SRS17204089 • All experiments • All runs
Name: Shot_CycDid_MNHN1998-234
Instrument: Illumina HiSeq 2500
Strategy: WGS
Selection: RANDOM
Layout: SINGLE
Runs: 1 run, 1.8M spots, 132M bases, 71.7Mb
Run# of Spots# of BasesSizePublished


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