Name: Kaiser_heterophil_control
Instrument: Illumina HiSeq 2500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: Macrophages and dendritic cells were cultured from bone marrow by standard methods (Garceau et al. 2010 and Wu et al. 2010, respectively), and heterophils isolated from blood by standard methods (Kogut et al. 1995). Cells were cultured with or without LPS for 24 h before RNA was isolated Standard Trizol extraction method Samples were prepared for mRNA sequencing using 2ug of total RNA starting material following the Illumina TruSeq RNA Sample Preparation v2 kit protocol.