Name: AM_SC4_1_s
Instrument: Illumina HiSeq 1500
Strategy: OTHER
Source: TRANSCRIPTOMIC SINGLE CELL
Selection: RT-PCR
Layout: SINGLE
Construction protocol: Lineage-positive cells were depleted from the bone marrow (BM) cell suspension by magnetic cell sorting using APC-Cyanine7 antibodies against CD3, CD19, Ly6G, NK1.1, Ter119 and anti-Cy7 beads (Miltenyi Biotech) according to the manufacturer's protocol followed by incubation with viability dyes and antibodies against cell surface markers diluted in FACS buffer (PBS, 2% FCS, 2 mM EDTA) for 20 minutes on ice in the dark. After washing with FACS buffer cells were resuspended in FACS buffer and sorted on a BD FACSAria Fusion as single cells into 96-well plates containing 5µl mcSCRB-seq lysis buffer (5M Guanidine HCl, 1% β-ME, 0.2% Phusion HF buffer 5x) per well. mRNA was extracted according to the mcSCRB-seq protocol (lysis buffer: 5M Guanidine HCl, 1% β-ME, 0.2% Phusion HF buffer 5x) ref: doi: 10.1038/s41467-018-05347-6 Libraries were generated according to the mcSCRB-seq protocol. ref: doi: 10.1038/s41467-018-05347-6