RNA-seq - delayed healing day 14 - SRA

SRX025782: RNA-seq - delayed healing day 14
1 ILLUMINA (Illumina Genome Analyzer II) run: 15.4M spots, 1.2G bases, 504.7Mb downloads

Design: Equal amounts of total RNA of 6 samples was pooled prior to library preparation. The library was prepared using the mRNA-Seq sample prep Kit (Illumina, San Diego, CA USA) according to the manufacturer’s instruction. Clusters were generated with Illumina’s v4 Single Read Cluster Generation Kit. The library was loaded onto one lane of the flow cell at 7 pM concentration. The flow cell was then sequenced on a Genome Analyzer IIx (Illumina, San Diego, CA, USA) for 76 cycles with v4 sequencing kits following the standard protocol and using SCS v2.6 software.

Submitted by: (Charité)

Study: Time series of standard and delayed bone healing in Ovis Aries

PRJNA79673 • SRP002632 • All experiments • All runs

show Abstracthide Abstract

Fracture healing is a highly complex regenerative process. The sheep is an important large-animal model for studying delayed fracture healing. Here we used next-generation sequencing (Illimuna GA IIx) for gene expression analysis (RNAseq) in two conditional groups - standard and delayed healing. In both groups sequential biopsies 7, 11, 14 and 21 days after surgery were collected from callus tissue and annalized. For all timepoints and conditions the samples were pooled (n=6), except for day 21 standard (n=5).

Sample: delayed healing day 14

SAMN00103508 • SRS107423 • All experiments • All runs

Organism: Ovis aries

Library:

Name: O-aries_callus_d14

Instrument: Illumina Genome Analyzer II

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: SINGLE

Spot descriptor:

1 forward

Runs: 1 run, 15.4M spots, 1.2G bases, 504.7Mb

Run	# of Spots	# of Bases	Size	Published
SRR064347	15,397,863	1.2G	504.7Mb	2011-04-26

ID:: 30306

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