GSM1817219: RNAseq_chimp1_rep2; Pan troglodytes; RNA-Seq - SRA

SRX1091570: GSM1817219: RNAseq_chimp1_rep2; Pan troglodytes; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2000) run: 46.5M spots, 9.3G bases, 4Gb downloads

Submitted by: Gene Expression Omnibus (GEO)

Study: Enhancer divergence and cis-regulatory evolution in the human and chimpanzee neural crest

PRJNA289483 • SRP060650 • All experiments • All runs

show Abstracthide Abstract

Here we derive human and chimpanzee cranial neural crest cells (CNCCs) and profile histone modifications, transcription factors, chromatin accessibility and gene expression to systematically and quantitatively annotate evolutionary divergence of craniofacial cis-regulatory landscapes. Overall design: Histone modifications (H3K27ac, H3K4me1, H3K4me3, H3K27me3), chromatin modifiers (p300), transcription factors (NR2F1, TFAP2A), chromatin accessibility (ATACseq) and gene expression (RNAseq) were assayed in CNCCs derived from iPSCs/ESCs from 2 chimpanzee and 3 human individuals.

Sample: RNAseq_chimp1_rep2

SAMN03854167 • SRS987157 • All experiments • All runs

Organism: Pan troglodytes

Library:

Instrument: Illumina HiSeq 2000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: ChIPs were performed using approximately 0.5-1 x 10^7 CNCCs fixed with 1% formaldehyde for 10 min at room temp. RNA was extracted using Trizol. Sequencing libraries were prepared starting from ~30ng of ChIP DNA or oligo-dT purified mRNA using the NEBNext Multiplex Oligos for Illumina kit (Cat# E7335S).

Experiment attributes:

GEO Accession: GSM1817219

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 46.5M spots, 9.3G bases, 4Gb

Run	# of Spots	# of Bases	Size	Published
SRR2096453	46,542,998	9.3G	4Gb	2015-09-11

ID:: 1597122

SRA

Sequence Read Archive

Result Filters

Send to:

Supplemental Content

Related information

Recent activity