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SRX109462: GSM840529: REV-ERB beta
1 ILLUMINA (Illumina Genome Analyzer II) run: 36.7M spots, 1.5G bases, 901.5Mb downloads

Submitted by: Gene Expression Omnibus (GEO)
Study: Integral roles for Rev-erb alpha and Rev-erb beta in the circadian clock function [ChIP_seq]
show Abstracthide Abstract
The circadian clock acts at the genomic level to coordinate internal behavioral and physiologic rhythms via the CLOCK-BMAL transcriptional heterodimer. Although the nuclear receptors REV-ERBa and ß have been proposed to contribute to clock function, their precise roles and importance remain unresolved. To establish their regulatory potential we generated comparative cistromes of both Rev-erb isoforms, which revealed shared recognition at over ~50% of their total sites and extensive overlap with the master clock regulator Bmal. While Rev-erba has been shown to directly regulate Bmal expression, the cistromic analysis reveals a more profound connection between Bmal and Rev-erba and ß regulatory circuits than previously suspected. Genes within the intersection of the Bmal and Rev-erb cistromes are highly enriched for both clock and metabolic functions. As predicted by the cistromic analysis, dual depletion of Rev-erba/ß function by creating double-knockout mice (DKOs) profoundly disrupted circadian expression of core clock and lipid homeostatic genes. As a result, DKOs show strikingly altered circadian wheel-running behavior and deregulated lipid metabolism. These data reveal an integral role of Rev-erba/ß in clock function as well as provide a cistromic basis for the integration of circadian rhythm and metabolism. Overall design: Identification of Reverb alpha and Reverb beta binding sites in mouse liver at ZT8
Sample: REV-ERB beta
SAMN00760895 • SRS281109 • All experiments • All runs
Organism: Mus musculus
Library:
Name: GSM840529: REV-ERB beta
Instrument: Illumina Genome Analyzer II
Strategy: ChIP-Seq
Source: GENOMIC
Selection: ChIP
Layout: SINGLE
Spot descriptor:
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Experiment attributes:
GEO Accession: GSM840529
Links:
External link:
Runs: 1 run, 36.7M spots, 1.5G bases, 901.5Mb
Run# of Spots# of BasesSizePublished
SRR38565136,651,2291.5G901.5Mb2012-04-02

ID:
126739

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