GSM1825056: Uninjected - rep 2; Xenopus tropicalis; RNA-Seq - SRA

SRX1099269: GSM1825056: Uninjected - rep 2; Xenopus tropicalis; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2000) run: 35.1M spots, 7G bases, 4.6Gb downloads

Submitted by: NCBI (GEO)

Study: Cdx ParaHox genes acquired distinct developmental roles after gene duplication in vertebrate evolution

PRJNA290093 • SRP061238 • All experiments • All runs

show Abstracthide Abstract

We examined the degree of regulatory and functional overlap between the three vertebrate Cdx genes using single and triple morpholino knock-down in Xenopus tropicalis followed by RNA-seq. Overall design: Stage 14 (early neurula) embryos derived from eggs injected with morpholinos against Cdx1, Cdx2, Cdx4 and a mixture of all three plus corresponding uninjected embryos. All in triplicates.

Sample: GEO accession GSM1825056 is currently private and is scheduled to be released on Jul 17, 2015.

SAMN03863247 • SRS993840 • All experiments • All runs

Organism: Xenopus tropicalis

Library:

Instrument: Illumina HiSeq 2000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: RNA was extracted with Tri-reagent (Sigma) and cleaned on Qiagen RNAeasy colmun according to manufacturer specification RNA libraries were prepared for sequencing using standard Illumina protocols

Experiment attributes:

GEO Accession: GSM1825056

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 35.1M spots, 7G bases, 4.6Gb

Run	# of Spots	# of Bases	Size	Published
SRR2105091	35,112,032	7G	4.6Gb	2015-07-20

ID:: 1604945

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