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SRX11122242: WGS of KSHV BAC16 Delete KapC
1 ILLUMINA (Illumina MiSeq) run: 348,591 spots, 167.7M bases, 85.4Mb downloads

Design: DNA was extracting using Qiagen Large Construct Kit. Libraries were prepared using a NEB ultra II DNA Library Prep Kit for Illumina and sequenced using a MiSeq 500 v2 nano run.
Submitted by: University of Calgary
Study: Kaposi's sarcoma-associated herpesvirus BAC16 kaposin-deficient viruses
show Abstracthide Abstract
The goal of this study overall was to create a panel of KSHV viruses targeting the polycistronic kaposin locus for deletion to enable characterization of individual protein function.The goal of this sequencing was to determine if recombination during mutagenesis had occurred as expected and that no off-target mutations arose.
Sample:
SAMN19672118 • SRS9185510 • All experiments • All runs
Library:
Name: KSHVdelC
Instrument: Illumina MiSeq
Strategy: WGS
Source: GENOMIC
Selection: RANDOM PCR
Layout: PAIRED
Runs: 1 run, 348,591 spots, 167.7M bases, 85.4Mb
Run# of Spots# of BasesSizePublished
SRR14789027348,591167.7M85.4Mb2021-06-11

ID:
14806614

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