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SRX11122252: WGS of KSHV BAC16
1 ILLUMINA (Illumina MiSeq) run: 498,606 spots, 243M bases, 118.8Mb downloads

Design: DNA was extracted using Large Construct Kit (Qiagen). DNA libraries were prepped using a NEB ultra II DNA Library Prep Kit for Illumina and sequenced using a MiSeq 500 v2 nano run.
Submitted by: University of Calgary
Study: Kaposi's sarcoma-associated herpesvirus BAC16 kaposin-deficient viruses
show Abstracthide Abstract
The goal of this study overall was to create a panel of KSHV viruses targeting the polycistronic kaposin locus for deletion to enable characterization of individual protein function.The goal of this sequencing was to determine if recombination during mutagenesis had occurred as expected and that no off-target mutations arose.
Sample:
SAMN19671530 • SRS9185528 • All experiments • All runs
Library:
Name: WT-KSHV-BAC16
Instrument: Illumina MiSeq
Strategy: WGS
Source: GENOMIC
Selection: RANDOM PCR
Layout: PAIRED
Runs: 1 run, 498,606 spots, 243M bases, 118.8Mb
Run# of Spots# of BasesSizePublished
SRR14789037498,606243M118.8Mb2021-06-11

ID:
14806624

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