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SRX12203443: single nucleus RNA-seq of mus musculus: Adult Heart Ventricles
1 ILLUMINA (NextSeq 550) run: 95.7M spots, 11.4G bases, 4.1Gb downloads

Design: Single nuclei were isolated from frozen ventricular chambers using differential centrifugation and filtration. Single nucleus RNA-seq libraries were constructed using 10X Genomics' NextGEM 3.1 3' kit according to manufacturer's instructions
Submitted by: Broad Institute
Study: SnRNA Sequencing Defines Signaling by RBC-Derived Extracellular Vesicles in the Murine Heart
show Abstracthide Abstract
Extracellular vesicles (EVs) are thought to mediate intercellular signaling by transferring their cargo to recipient cells. Red blood cell (RBC)-derived EVs constitute a significant proportion of circulating EVs and have been implicated in regulating immune responses. Here, we describe the use of a transgenic mouse model for fluorescence-based mapping of RBC-EV recipient cells to assess the functional role of this intercellular signaling mechanism in the pathogenesis of ventricular remodeling after ischemic injury. In this model of ischemic heart failure, we detected an increase in RBC-EV-targeted cardiomyocytes in the heart by fluorescent-based mapping. Single cell nuclear RNA sequencing of the heart revealed a complex landscape of cardiac cells targeted by RBC-EVs, suggesting enriched expression of genes implicated in cell proliferation and metabolism pathways compared to non-targeted cells. Correspondingly, cardiomyocytes targeted by RBC-EVs are more likely to express cellular markers of DNA synthesis and proliferation, suggesting the functional significance of EV-mediated signaling. In conclusion, our mouse model for mapping of EV-recipient cells reveals a complex network of RBC-EV mediated intercellular communication in ischemic heart failure and suggests a functional role for this mode of intercellular signaling.
Sample: Single nucleus RNA sequencing from ventricular chambers
SAMN21450746 • SRS10193229 • All experiments • All runs
Organism: Mus musculus
Name: IR_297
Instrument: NextSeq 550
Strategy: RNA-Seq
Selection: Oligo-dT
Layout: PAIRED
Runs: 1 run, 95.7M spots, 11.4G bases, 4.1Gb
Run# of Spots# of BasesSizePublished


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