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SRX12488732: GSM5610938: WT_replicate3; Drosophila melanogaster; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2000) run: 45.1M spots, 2.3G bases, 1.4Gb downloads

External Id: GSM5610938_r1
Submitted by: IRCM, Inserm
Study: RNA-Seq of Notch-stimulated overgrowing Drosophila melanogaster wing imaginal discs
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Aim: mRNA profile of larval wing imaginal discs of Drosophila melanogaster to study the cooperation between Notch activation and loss of epithelial polarity (scrib mutation) during neoplastic growth. Results: The combination of Notch activation and scribble mutation (NS) results in mRNA expression changes that, while partly overlapping with Notch only (N), and with scrib mutation only (S), are unique to the combination Overall design: Triplicates of pooled dissected 3rd instar larva wing imaginal discs (60 discs in each pool). All dissections at day 6 after egg laying for the following conditions: wild type (WT), activated Notch (N), polarity deficient (scrib1 mutant, S), activated Notch and polarity deficient (NS)
Sample: WT_replicate3
SAMN22063112 • SRS10451921 • All experiments • All runs
Name: GSM5610938
Instrument: Illumina HiSeq 2000
Strategy: RNA-Seq
Selection: cDNA
Layout: SINGLE
Construction protocol: 60 wing imaginal discs were dissected in cold PBS and pooled. Total RNA was extracted using Trizol and genomic DNA was removed using Ambion's DNA-free kit (#AM 1906). Illumina TruSeq Stranded mRNA Sample Prep Kit was used with 1 ug of total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols
Runs: 1 run, 45.1M spots, 2.3G bases, 1.4Gb
Run# of Spots# of BasesSizePublished


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