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SRX12566825: GSM5620872: Control_sample_rep2; Drosophila melanogaster; RNA-Seq
1 ILLUMINA (NextSeq 500) run: 30.7M spots, 4.9G bases, 1.9Gb downloads

Submitted by: NCBI (GEO)
Study: Gene expression profiling of Drosophila melanogaster larval brains after chronich alcohol exposure
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We sequenced mRNA extracted from brains of (1) D. melanogaster larvae exposed to food containing 5% ethanol (v/v) for 6 conscutive days, and (2) an age-matched untreated control larvae, that grew in regular food. Differential gene expression between the two groups was calculated and reported. Each group consisted of 3 biological replicates of 30 brains each. Overall design: Examination of mRNA levels in brains of D. melanogaster larvae after chronich ethanol exposure was performed using next generation sequencing (NGS) technology (RNA-seq).
Sample: Control_sample_rep2
SAMN22208868 • SRS10524066 • All experiments • All runs
Instrument: NextSeq 500
Strategy: RNA-Seq
Selection: cDNA
Layout: PAIRED
Construction protocol: Six days after egg-laying, as larvae began to crawl out of the food to proceed with pupae formation, individual third-instar larvae were collected using a needle, transferred to microcentrifuge tubes, and frozen at -80°C for 24 to 48 hours. Freezing was followed by the addition of 200uL of RNAlater-ICE Frozen Tissue Transition Solution (AM7030, Thermo Fisher Scientific) to larvae. Approximately 30 larval brains were dissected per replicate in RNAlater-ICE, for a total of three replicates (~90 larval brains) for each group. Total RNA was extracted from larval brains using EZ1 RNA Tissue mini kit (EZ1 RNA Tissue Mini Kit no. 959034, Qiagen). All samples were treated with DNase I (DNase Set no. 79254, Qiagen). RNA concentration and quality were evaluated using a Qubit 2.0 fluorometer (Life Technologies, USA) and Agilent 2100 Bioanalyzer (Agilent Technologies, USA). Capture of mRNA (poly-A selection) and library preparation was carried out using the Illumina TruSeq® Stranded mRNA Library Prep kit from 300ng of total RNA. Libraries were sequenced with an Illumina NextSeq 500/550 High Output Kit v2.5 for 80 cycles following manufacturer's protocol (Illumina, Inc). At least 20 million reads were obtained for each replicate (a pool of 30 brains).
Experiment attributes:
GEO Accession: GSM5620872
Runs: 1 run, 30.7M spots, 4.9G bases, 1.9Gb
Run# of Spots# of BasesSizePublished


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