RNA-Seq of Crassostrea gigas: trochophore warmed rep2 - SRA

SRX1331737: RNA-Seq of Crassostrea gigas: trochophore warmed rep2
1 ILLUMINA (Illumina HiSeq 2000) run: 22.7M spots, 4.6G bases, 2.7Gb downloads

Design: "Samples consisted of approximately 50,000 pooled larvae, and were collected by filtration from experimental tanks at 14 (trochophore), 24 (early veliger) and 36 (late veliger) hours post fertilization for each of the four treatments (control = 20°C pH 8.1; acidified = 20°C pH 7.9; warmed = 22°C pH 8.1; interaction = 22°C pH 7.9). Samples were flash frozen in liquid nitrogen and stored at -80°C until RNA extraction. Samples were homogenised with steel beads and total RNA was extracted from two replicate samples per treatment per time point using TRIzol reagent (Life Technologies) according to manufacturer's instructions. RNA quality and concentration were determined using an Agilent 2100 RNA Bioanalyser (Agilent, Santa Clara, CA, USA) and NanoDrop 8000 Spectrophotometer (NanoDrop Technologies, Wilmington, DE, USA) respectively. Library preparation and sequencing with illumina HiSeq 2000 (100 bp paired-end) was carried out at the Institute of Clinical Molecular Biology, Christian-Albrechts-University Kiel, Germany, with 8 samples multiplexed per lane."

Submitted by: University of Brest

Study: Crassostrea gigas Transcriptome or Gene expression

PRJNA298285 • SRP064660 • All experiments • All runs

show Abstracthide Abstract

Transcriptome of the Pacific oyster Crassostrea gigas, comprising larval tissue exposed to two pHs and two temperatures.

Sample: Pooled samples of 50000 larvae

SAMN10261165 • SRS3959412 • All experiments • All runs

Organism: Crassostrea gigas

Library:

Name: C3765_Trc_warmB

Instrument: Illumina HiSeq 2000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: RANDOM

Layout: PAIRED

Spot descriptor:

1 forward101 reverse

Runs: 1 run, 22.7M spots, 4.6G bases, 2.7Gb

Run	# of Spots	# of Bases	Size	Published
SRR2601709	22,670,096	4.6G	2.7Gb	2015-10-13

ID:: 1897184

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