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SRX1451466: HL-60/S4 TPA treated cells, replicate 1
1 ILLUMINA (Illumina HiSeq 2000) run: 78.9M spots, 15.9G bases, 9.5Gb downloads

Design: Cells were exposed to 16nmaol/L tetradecanoyl phorbol acetate for 4 days. Total RNA was extracted from 1x10^6 cells using the Qiagen QIAshredder spin column kit then purified with an RNeasy MinElute column. The RNA-Seq library was prepared using Illumina the TruSeq RNA Library Preparation Kit v2 after shearing 1ug of total RNA to ~175nt. Library quality was validated on a Agilent Bioanalyzer and Qubit fluorometer.
Submitted by: Marine Biological Laboratory
Study: Comparing The Transcriptomes of Granulocytic and Macrophage Differentiated Forms of HL-60/S4 Cells
show Abstracthide Abstract
The acute myeloblastic leukemia cell line HL-60 can be differentiated in vitro from a rapidly growing promyelocytic form to a nongrowing form, resembling neutrophils with segmented (lobulated) nuclei, by the addition of retinoic acid. Treating the undifferentiated cells with phorbol ester (TPA) leads to rapid cessation of cell division and attachment of the treated cells to culture dishes, exhibiting characteristics of macrophage. The rapidly differentiating subline HL-60/S4 has been used to study nuclear shape, chromatin structure and cytoskeletal changes during differentiation induced by RA and TPA. This study seeks to understand phenotypic properties of the differentiated granulocyte and macrophage forms of HLA-60/S4 by examining through RNA-Seq the transcriptional differences between RA- TPA- and untreated cells 4 days after exposure.
Sample: HL-60/S4 cells 4 days after exposure to phorbol ester
SAMN04293274 • SRS1179361 • All experiments • All runs
Organism: Homo sapiens
Name: HL60S4_TPA-1
Instrument: Illumina HiSeq 2000
Strategy: RNA-Seq
Selection: cDNA
Layout: PAIRED
Spot descriptor:
forward102  reverse

Runs: 1 run, 78.9M spots, 15.9G bases, 9.5Gb
Run# of Spots# of BasesSizePublished


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