Comprehensive transcriptome analyses of the fructose-fed Syrian golde... - SRA

SRX1737626: Comprehensive transcriptome analyses of the fructose-fed Syrian golden hamster liver provides novel insights into lipid metabolism
1 ILLUMINA (Illumina HiSeq 2000) run: 26.5M spots, 1.3G bases, 905.6Mb downloads

Design: Liver tissues were homogenized into a fine powder before RNA extraction. Total RNA was extracted using the RNeasy Mini Kit (Qiagen) according to the manufacturer’s instructions, including treatment with DNase. RNA quality was evaluated with an Agilent 2100 Bioanalyzer (Agilent RNA 6000 pico & DNA 1000 Kit). All RNA samples had an RNA integrity number (RIN) no less than 7.5. Sequencing libraries were prepared using the Illumina TruSeq RNA-Seq library protocol coupled with a Poly-A protocol according to the manufacturer’s instructions.

Submitted by: Wenzhou Medical University

Study: Mesocricetus auratus Raw sequence reads

PRJNA320146 • SRP074216 • All experiments • All runs

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In this study, we performed whole transcriptome analysis of fructose-fed Syrian golden hamster liver based on the next-generation sequencing technology to uncover the alteration of coding genes, lncRNAs and miRNAs expression profile after fructose-fed which could help to illuminate the molecular mechanisms of VLDL synthesis from the transcription levels.

Sample:

SAMN04917437 • SRS1418178 • All experiments • All runs

Organism: Mesocricetus auratus

Library:

Instrument: Illumina HiSeq 2000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: RANDOM

Layout: SINGLE

Spot descriptor:

1 forward

Runs: 1 run, 26.5M spots, 1.3G bases, 905.6Mb

Run	# of Spots	# of Bases	Size	Published
SRR3468375	26,466,896	1.3G	905.6Mb	2017-04-30

ID:: 2487556

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