Zebrafish pineal gland CT22b - SRA

SRX195406: Zebrafish pineal gland CT22b
1 ILLUMINA (Illumina HiSeq 2000) run: 17M spots, 1.7G bases, 1Gb downloads

Design: library construction was conducted using Illumina's TruSeq RNA Sample Prep Kit following the manufacture protocol. Briefly, starting from total RNA, the messenger RNA was purified using polyA selection, then chemically fragmented and converted into single-stranded cDNA using random hexamer priming. Afterwards, the second strand was generated to create double-stranded cDNA. Finally, adapters were added by ligation to the double-stranded cDNA, which prepared the samples for cluster generation. After library quality check by Bioanalyzer High Sensitivity chip, clusters were generated and libraries were run on HiSeq2000 by using standard Illumina sequencing workflow with the multiplexing option. The mRNA libraries from the zebrafish pineal glands were sequenced on two lanes of Illumina HiSeq2000 instrument following the manufacture protocol. In each sequencing lane six bar-codes were used, one for each tested sample. Primary data analyses of sequence data and quality controls were performed using the Illumina pipeline (version 1.7). The reads were filtered using Illumina's HiSeq2000 softwares, Illumina indexes separation was also performed.

Submitted by: TEL AVIV UNIVERSITY

Study: Danio rerio Transcriptome or Gene expression

PRJNA177642 • SRP016132 • All experiments • All runs

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Pineal glands of adult zebrafish were sampled every 4 hours for 48 hours.

Sample: Zebrafish pineal gland

SAMN01765705 • SRS369337 • All experiments • All runs

Organism: Danio rerio

Library:

Instrument: Illumina HiSeq 2000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Spot descriptor:

1 forward52 reverse

Runs: 1 run, 17M spots, 1.7G bases, 1Gb

Run	# of Spots	# of Bases	Size	Published
SRR592706	16,983,954	1.7G	1Gb	2012-12-14

ID:: 258417

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