GSM2477888: granulosa cells sample 3; Bos taurus; RNA-Seq - SRA

SRX2539721: GSM2477888: granulosa cells sample 3; Bos taurus; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2500) run: 43M spots, 8.6G bases, 2.9Gb downloads

Submitted by: NCBI (GEO)

Study: Identification of potential biomarkers in donor cows for in vitro embryo production by granulosa cells transcriptomics

PRJNA371462 • SRP098909 • All experiments • All runs

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In this study we sequenced RNA of granulosa collected from Holstein cows at oocyte aspiration prior to IVP, to identify candidate genes and biological mechanisms for favourable IVP-related traits in donor cows. Overall design: 24 RNA-Seq profiles of granulosa cells collected from antral follicles of 24 cows. Each animal was scored for In Vitro Production performances (blastocysts rate, morphology and kinetic scores). Half of the oocytes were inseminated with semen from high genetic merit bulls and half with semen from low genetic merit bulls.

Sample: granulosa cells sample 3

SAMN06299491 • SRS1959433 • All experiments • All runs

Organism: Bos taurus

Library:

Instrument: Illumina HiSeq 2500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: RNA was isolated with RNeasy Plus Micro Kit (Qiagen) Illumina TruSeq-stranded totalRNA RiboZero Gold Prep Kit

Experiment attributes:

GEO Accession: GSM2477888

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 43M spots, 8.6G bases, 2.9Gb

Run	# of Spots	# of Bases	Size	Published
SRR5231247	42,954,375	8.6G	2.9Gb	2017-04-03

ID:: 3676312

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