RNAseq of water buffalo mammary carcinoma - SRA

SRX2753520: RNAseq of water buffalo mammary carcinoma
1 ILLUMINA (Illumina HiSeq 2500) run: 19.2M spots, 1.9G bases, 828Mb downloads

Design: Illumina HiSeq 2500, Paired end run; 2 X 100 bp. ~5-10 ug of total RNA was used to prepare the RNA seq library using the TruSeq RNA Sample Prep Kits (Illumina). The libraries were prepared as per the kit protocol. Following purification, the mRNA was fragmented into small pieces using divalent cations under elevated temperature. The cleaved mRNA fragments was used to synthesize first strand cDNA synthesis using DNA polymerase I and RNaseH. These cDNA then proceed through an end repair process, the addition of a single 'A' base and ligation of adapters. The prodcuts were purified and enriched with PCR to create the final cDNA library.

Submitted by: Guru Angad Dev Veterinary and Animal Sciences University

Study: Bubalus bubalis Raw sequence reads

PRJNA383059 • SRP104171 • All experiments • All runs

show Abstracthide Abstract

Goal of the experiment was to know the gene networks and pathways that are operating in buffalo mammary carcinoma. Rarity of ruminant mammary cacinoma makes this study relevant from agriculture point of view.

Sample: Mammay tissue samples from Bubalus bubalis

SAMN06758773 • SRS2130861 • All experiments • All runs

Organism: Bubalus bubalis

Library:

Name: BUMG_39_2

Instrument: Illumina HiSeq 2500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: RANDOM PCR

Layout: PAIRED

Runs: 1 run, 19.2M spots, 1.9G bases, 828Mb

Run	# of Spots	# of Bases	Size	Published
SRR5468164	19,228,969	1.9G	828Mb	2018-09-18

ID:: 3970097

SRA

Sequence Read Archive

Result Filters

Send to:

Supplemental Content

Related information

Recent activity