GSM1298098: st10.5 FoxH1 MO; Xenopus tropicalis; RNA-Seq - SRA

SRX399455: GSM1298098: st10.5 FoxH1 MO; Xenopus tropicalis; RNA-Seq
1 ILLUMINA (Illumina Genome Analyzer IIx) run: 20.6M spots, 782.6M bases, 554.9Mb downloads

Submitted by: NCBI (GEO)

Study: Genome-wide view of TGFb/Foxh1 regulation of the early mesendoderm program [RNA-seq]

PRJNA232590 • SRP034731 • All experiments • All runs

show Abstracthide Abstract

We identified Nodal and Foxh1 downstream targets by performing RNA-seq of embryos either treated with small molecule SB431542 or microinjected morpholino anti-sense oligo against Foxh1. Overall design: Differential gene expression analyses of perturbed embryos (SB431542 treated, or Foxh1 MO injected) using RNA-seq

Sample: st10.5 FoxH1 MO

SAMN02486399 • SRS519889 • All experiments • All runs

Organism: Xenopus tropicalis

Library:

Instrument: Illumina Genome Analyzer IIx

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: SINGLE

Construction protocol: Total RNAs were extracted from ~25-30 control and experimental early gastrula embryos using the acid guanidinium thiocyanate phenol chloroform method (Chomczynski and Sacchi 1987), followed by selective precipitation of RNA using 2.5M LiCl. Libraries were prepared using illumina TruSeq™ RNA Kit

Experiment attributes:

GEO Accession: GSM1298098

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 20.6M spots, 782.6M bases, 554.9Mb

Run	# of Spots	# of Bases	Size	Published
SRR1060752	20,594,222	782.6M	554.9Mb	2015-07-22

ID:: 573898

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