Western Corn Rootworm (Diabrotica virgifera virgifera) transcriptome ... - SRA

SRX426241: Western Corn Rootworm (Diabrotica virgifera virgifera) transcriptome sequence from third instar midgut tissues
1 LS454 (454 GS FLX Titanium) run: 1.2M spots, 355.8M bases, 236.3Mb downloads

Design: For 454 EST sequencing WCR third-instar midgut tissues were isolated from 200 larvae and stored in RNAlater (Life Technologies) prior to extraction. Total RNA extraction was performed on four sets of 50 midguts using the RNeasy mini kit (Qiagen). Approximately 500 μg of total RNA were used for two rounds of PolyA selection using the MicroPoly(A)Purist kit (Life Technologies). Following PolyA selection, cDNAs were synthesized using the SuperScript II cDNA synthesis kit (Life Technologies), using random hexamers for priming during first-strand synthesis. Finally, cDNAs were purified using a QIAquick PCR purification column (Qiagen). Before 454-FLX sequencing, emulsion PCR reactions were conducted following the manufacturers specifications (Roche).

Submitted by: (MONSANTO)

Study: Diabrotica virgifera virgifera strain:Waterman Random survey

PRJNA234446 • SRP035364 • All experiments • All runs

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EST collection from Western Corn Rootworm third instar midgut tissues

Sample: whole genome sequence of pooled population (5 animals) from Waterman IL USA

SAMN02373849 • SRS528965 • All experiments • All runs

Organism: Diabrotica virgifera virgifera

Library:

Instrument: 454 GS FLX Titanium

Strategy: EST

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: SINGLE

Spot descriptor:

5 forward

Runs: 1 run, 1.2M spots, 355.8M bases, 236.3Mb

Run	# of Spots	# of Bases	Size	Published
SRR1187860	1,202,023	355.8M	236.3Mb	2014-04-02

ID:: 602864

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