GSM4096273: TATRK_Rep2; Rattus norvegicus; RNA-Seq - SRA

SRX6902879: GSM4096273: TATRK_Rep2; Rattus norvegicus; RNA-Seq
1 ILLUMINA (Illumina HiSeq 4000) run: 17.1M spots, 5.1G bases, 2Gb downloads

Submitted by: NCBI (GEO)

Study: Inhibition of the Dead Box RNA Helicase 3 prevents HIV-1 Tat and cocaine-induced neurotoxicity by targeting microglia activation

PRJNA574107 • SRP223249 • All experiments • All runs

show Abstracthide Abstract

We have investigated the protective effect of DDX3 inhibitor RK33 on toxic damage done by combined TAT and cocaine. Overall design: Primary neuronal cultures were treated with HIV-protein TAT and cocaine following RK33 treatment. Each treatment condition was done in biological triplicate.

Sample: TATRK_Rep2

SAMN12840107 • SRS5434776 • All experiments • All runs

Organism: Rattus norvegicus

Library:

Instrument: Illumina HiSeq 4000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: Total RNA was extracted using Quick-RNA MicroPrep Kit(ZYMO) and RNA quality was validated on RNA-1000 chip using Bioanalyzer (Agilent) Sequencing libraries were generated using the NEBNext Ultra II Directional RNA Library Prep Kit(NEB#E7490/NEB#E7760)

Experiment attributes:

GEO Accession: GSM4096273

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 17.1M spots, 5.1G bases, 2Gb

Run	# of Spots	# of Bases	Size	Published
SRR10182259	17,121,345	5.1G	2Gb	2019-10-09

ID:: 9082027

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