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SRX7427169: GSM4230182: female normoxia medulla replicate 4 [FNV_B24]; Rattus norvegicus; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2500) run: 22.6M spots, 3.4G bases, 988Mb downloads

Submitted by: NCBI (GEO)
Study: Sex- and region-specific transcriptomic differences in brainstem and cervical spinal cord microglia in rat offspring exposed to gestational intermittent hypoxia
show Abstracthide Abstract
We analyzed gene expression via RNA-sequencing in medulla and C3-C6 cervical spinal cord microglia isolated from young adult rats exposed to gestational intermittent normoxia or gestational intermittent hypoxia Overall design: G10 sprague dawley rat dams were exposed to one of the following conditions: 1) 11 days of intermittent normoxia (21%/21% O2) or 2) 11 days of intermittent hypoxia (21%/10.5% O2). Offspring were born under normoxic conditions and allowed to grow to 8 weeks. Medulla and C3-C6 cervical spinal cord from 1 male and 1 female from 5 paired litters were harvested. Microglia were immunomagnetically isolated from each region and total RNA isolated for sequencing.
Sample: female normoxia medulla replicate 4 [FNV_B24]
SAMN13663600 • SRS5873254 • All experiments • All runs
Library:
Instrument: Illumina HiSeq 2500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: SINGLE
Construction protocol: Microglia were immunomagentically isolated using Miltenyi anti-cd11b antibody. Microglia were lysed in trizol. RNA libraries were prepared for sequencing using standard Illumina protocols
Experiment attributes:
GEO Accession: GSM4230182
Links:
Runs: 1 run, 22.6M spots, 3.4G bases, 988Mb
Run# of Spots# of BasesSizePublished
SRR1075236722,576,9083.4G988Mb2020-07-13

ID:
9719004

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