GSM1531689: WT-F-Ov; Oryzias latipes; RNA-Seq - SRA

SRX742779: GSM1531689: WT-F-Ov; Oryzias latipes; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2000) run: 26.8M spots, 4.8G bases, 3.1Gb downloads

Submitted by: Gene Expression Omnibus (GEO)

Study: Transcriptomic characterization of transcription activator-like effector nucleases-induced XY dmy- sex reversal female medaka (Oryzias latipes)

PRJNA264833 • SRP049254 • All experiments • All runs

show Abstracthide Abstract

We assembled transcription activator-like effector nucleases (TALENs) targeting the DMY gene and generated XYdmy- mutants to investigate gonadal dysgenesis in medaka. RNA-seq generated 157 million raw reads from WT male (WT_M_TE), WT female (WT_F_OV) and XYdmy- female medaka (TA_F_OV) gonad libraries. Differential expression analysis identified 144 up- and 293 downregulated genes in TA_F_OV compared to WT_F_OV, 387 up- and 338 downregulated genes in TA_F_OV compared to WT_M_TE. Overall design: mRNA profiles of 90-day old wild type (WT) and TALENs induced XY dmy- sex reversal female (TA) medaka were generated by deep sequencing, using Illumina HiSeqTM 2000.

Sample: WT-F-Ov

SAMN03142905 • SRS730486 • All experiments • All runs

Organism: Oryzias latipes

Library:

Instrument: Illumina HiSeq 2000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: After the total RNA extraction, magnetic beads with Oligo (dT) are used to isolate mRNA(for eukaryotes). Mixed with the fragmentation buffer, the mRNA is fragmented into short fragments. cDNA is synthesized using the mRNA fragments as templates. Short fragments are purified and resolved with EB buffer for end reparation and single nucleotide A addition. After that, the short fragments are connected with adapters.The suitable fragments are selected for the PCR amplification as templates. During the QC steps, Agilent 2100 Bioanaylzer and ABI StepOnePlus Real-Time PCR System are used in quantification and qualification of the sample library.The library could be sequenced using Illumina HiSeq™ 2000

Experiment attributes:

GEO Accession: GSM1531689

Links:

External link: GEO Sample GSM1531689

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 26.8M spots, 4.8G bases, 3.1Gb

Run	# of Spots	# of Bases	Size	Published
SRR1628838	26,814,007	4.8G	3.1Gb	2017-02-13

ID:: 1073656

SRA

Sequence Read Archive

Result Filters

Send to:

Supplemental Content

Related information

Recent activity