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SRX7443232: GSM4233274: scRNA 3; Homo sapiens; RNA-Seq
1 ILLUMINA (Illumina HiSeq 4000) run: 60.8M spots, 16.8G bases, 6Gb downloads

Submitted by: NCBI (GEO)
Study: Transcriptome of Human Primary Corneal Endothelial Cells with SLC4A11 deficiency
show Abstracthide Abstract
Mutations in the solute-linked carrier family 4 member 11 (SLC4A11) gene are associated with several corneal endothelial dystrophies, in all of which visually significant cornea edema may require corneal transplantation. To elucidate the pathogenesis of SLC4A11 associated corneal endothelial dystrophies, we analyzed the transcriptome of SLC4A11 knock-down primary human corneal endothelium (SLC4A11 KD pHCEnC) and scrambled RNA treated pHCEnC as controls. Overall design: 3 KD vs 3 CTRL
Sample: scRNA 3
SAMN13683643 • SRS5887530 • All experiments • All runs
Organism: Homo sapiens
Library:
Instrument: Illumina HiSeq 4000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: Total RNA from cultured pHCEnC was isolated in TRI Reagent and purified with the RNeasy Clean-Up Kit (Qiagen). Purified total RNA from pHCEnC was prepared for RNA-seq libraries using KAPA mRNA HyperPrep Kit.
Experiment attributes:
GEO Accession: GSM4233274
Links:
Runs: 1 run, 60.8M spots, 16.8G bases, 6Gb
Run# of Spots# of BasesSizePublished
SRR1076931460,803,18516.8G6Gb2020-08-13

ID:
9735121

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