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SRX7443233: GSM4233275: SLC4A11 KD 1; Homo sapiens; RNA-Seq
1 ILLUMINA (Illumina HiSeq 4000) run: 65.3M spots, 18.1G bases, 6.5Gb downloads

Submitted by: NCBI (GEO)
Study: Transcriptome of Human Primary Corneal Endothelial Cells with SLC4A11 deficiency
show Abstracthide Abstract
Mutations in the solute-linked carrier family 4 member 11 (SLC4A11) gene are associated with several corneal endothelial dystrophies, in all of which visually significant cornea edema may require corneal transplantation. To elucidate the pathogenesis of SLC4A11 associated corneal endothelial dystrophies, we analyzed the transcriptome of SLC4A11 knock-down primary human corneal endothelium (SLC4A11 KD pHCEnC) and scrambled RNA treated pHCEnC as controls. Overall design: 3 KD vs 3 CTRL
Sample: SLC4A11 KD 1
SAMN13683642 • SRS5887531 • All experiments • All runs
Organism: Homo sapiens
Instrument: Illumina HiSeq 4000
Strategy: RNA-Seq
Selection: cDNA
Layout: PAIRED
Construction protocol: Total RNA from cultured pHCEnC was isolated in TRI Reagent and purified with the RNeasy Clean-Up Kit (Qiagen). Purified total RNA from pHCEnC was prepared for RNA-seq libraries using KAPA mRNA HyperPrep Kit.
Experiment attributes:
GEO Accession: GSM4233275
Runs: 1 run, 65.3M spots, 18.1G bases, 6.5Gb
Run# of Spots# of BasesSizePublished


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