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SRX858064: GSM1595913: rhesus_monkey_spleen; Macaca mulatta; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2000) run: 49.8M spots, 9G bases, 5.5Gb downloads

Submitted by: Gene Expression Omnibus (GEO)
Study: Transcriptome study in rhesus monkey
show Abstracthide Abstract
RNA-Seq technology was widely used to study gene expression, splicing structure and RNA editing. To facilitate transcriptome study in rhesus macaque, and provide an evolutionary context to understand human biology, we performed 90bp × 2, paired-end, strand-specific, polyA-postive RNA-Seq in 10 tissues of the same macaque animals. The data were deposited under accession number GSE42857 and GSE34426. Overall design: This submission represents RNA-Seq: cerebellum, lung, kidney, heart and spleen component of study.
Sample: rhesus_monkey_spleen
SAMN03316965 • SRS829896 • All experiments • All runs
Organism: Macaca mulatta
Library:
Instrument: Illumina HiSeq 2000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: Total RNA was extracted from rhesus monkey tissues using the Trizol method and analyzed by an Agilent 2100 bio-analyzer (Agilent Technologies). Poly (A)-positive RNA was purified with the Dynabeads mRNA purification kit (Invitrogen) from 5μg of total RNA with high quality (RNA integrity number >7.5), following the manufacturer's instructions. A strand-specific RNA-Seq library preparation on the basis of the incorporation of deoxy-UTP was performed.
Experiment attributes:
GEO Accession: GSM1595913
Links:
External link:
Runs: 1 run, 49.8M spots, 9G bases, 5.5Gb
Run# of Spots# of BasesSizePublished
SRR177844149,847,4019G5.5Gb2015-01-31

ID:
1228266

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