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SRX9402647: GSM4873706: ATAC_20h_Bioreplicate 1.techinical replicate 2; Strongylocentrotus purpuratus; ATAC-seq
1 ILLUMINA (Illumina HiSeq 2500) run: 71.3M spots, 5.4G bases, 1.7Gb downloads

Submitted by: NCBI (GEO)
Study: PRO-seq, ATAC-seq, and Pol II ChIP-seq in 20 hour sea urchin embryos (ATAC-Seq)
show Abstracthide Abstract
PRO-seq, ATAC-seq, and Pol II ChIP-seq signals are used to predict active developmental enhancers. Overall design: Machine learning models are trained and tested wit the different epigenetic marks for the precdiction of genomic with active reporter gene activity.
Sample: ATAC_20h_Bioreplicate 1.techinical replicate 2
SAMN16597773 • SRS7620517 • All experiments • All runs
Library:
Instrument: Illumina HiSeq 2500
Strategy: ATAC-seq
Source: GENOMIC
Selection: other
Layout: SINGLE
Construction protocol: Nuclear prep, Hajdu et al., 2016. PMID: 27896813 Wash nuclei once with Cell lysis buffer (10 mM Tris-HCl, pH 7.4, 10 mM NaCl, 3 mM MgCl2, 0.1% IGEPAL CA-630) Nextera DNA Sample Preparation Kit (24 Samples) (Illumina Cat # FC-121-1030) was used for the transposase reaction. Nextera DNA Sample Preparation Kit (24 Samples) (Illumina Cat # FC-121-1030) was used for library generation
Experiment attributes:
GEO Accession: GSM4873706
Links:
Runs: 1 run, 71.3M spots, 5.4G bases, 1.7Gb
Run# of Spots# of BasesSizePublished
SRR1293916471,285,3995.4G1.7Gb2021-06-03

ID:
12273634

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