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SRX9530669: small RNA-Seq of Tribolium castaneum after heat stress with 1 hour recovery
1 ILLUMINA (NextSeq 500) run: 70.2M spots, 3.6G bases, 2Gb downloads

Design: Libraries were prepared using the NEBNext Multiplex Small RNA Library Prep Set for Illumina starting with 450ng of input RNA and 13 cycles of amplification PCR. The libraries were pooled equimolar in a 6-plex pool, which was loaded to a single lane of a HiSeq2500 sequencing instrument at 6 pM concentration and was sequenced in a 50 single-read mode.
Submitted by: Ruder Bodkovic Institute
Study: Satellite DNA in gene regulation and environmental adaptation
show Abstracthide Abstract
The study aimed at investigating the influence of heat stress on satellite DNA analyzed with RNA-Seq and small RNA-Seq in beetle Tribolium castaneum
Sample:
SAMN16833512 • SRS7737250 • All experiments • All runs
Library:
Name: tca5
Instrument: NextSeq 500
Strategy: miRNA-Seq
Source: TRANSCRIPTOMIC
Selection: size fractionation
Layout: SINGLE
Runs: 1 run, 70.2M spots, 3.6G bases, 2Gb
Run# of Spots# of BasesSizePublished
SRR1308396770,211,3983.6G2Gb2020-11-19

ID:
12453622

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