Name: GSM5717666
Instrument: Illumina NovaSeq 6000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: Small pieces of tissues were incubated with 1 mL of 0.4 mg/mL of Liberase TL (Roche, 05401020001) in DMEM with 100 units/ml of penicillin, 100 mg/ml of streptomycin and washed with PBS then incubated with 0.05% TrypLE™ Select. Cell suspension was washed with 10 % FBS in DMEM with 100 units/ml of penicillin, 100 mg/ml of streptomycin then incubated with 100 Unit/ml of DNAse I (New England BioLabs, M0303). Cell suspension was washed with 0.04 (w/v) % BSA in PBS and cell suspension was prepared by filtering through 40 mm strainer. To achieve live single cell suspension, Propidium Iodide (BioLegend, 421301) negative and Vybrant DyeCycle Violet (Invitrogen, V35003) positive single cells were sorted. Library construction was 10x Chromium Single Cell v3. Sequencing was performed on the NovaSeq 6000, v1.5 reagents. Basecalling and sample demultiplexing is performed within Illumina's BaseSpace software.