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SRX5167560: GSM3521716: kdrl:GFP+, 72 hpf; Danio rerio; RNA-Seq
4 ILLUMINA (Illumina HiSeq 2500) runs: 33.4M spots, 1.9G bases, 861.5Mb downloads

Submitted by: NCBI (GEO)
Study: Transcription factor induction of vascular blood stem cell niches in vivo [scRNA-Seq]
PRJNA510839 • SRP173970 • All experiments • All runs
show Abstracthide Abstract
We report single cell gene expression data for FACS-isolated zebrafish kdrl(flk1):GFP positive cells collected from whole embryos at 72 hours post fertilization (hpf). Overall design: kdrl:GFP positive transgenic embryos were homogenized, filtered, and then 25,000 GFP+ cells were FACS sorted into PBS. 2,000 cells were then encapsulated using the inDrops method and libraries were prepared for sequencing.
Sample: kdrl:GFP+, 72 hpf
SAMN10617104 • SRS4175445 • All experiments • All runs
Organism: Danio rerio
Library:
Instrument: Illumina HiSeq 2500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: SINGLE
Construction protocol: Approximately 2,000 kdrl:GFP+ cells were encapsulated using the inDrops method (Zilionis et al., 2017). Libraries were prepared for sequencing as previously described. Libraries were prepared as previously described (Zilionis et al., 2017) and sequenced on an Illumina Hiseq 2500 Single cell RNA-seq (inDrops)
Experiment attributes:
GEO Accession: GSM3521716
Links:
Runs: 4 runs, 33.4M spots, 1.9G bases, 861.5Mb
Run# of Spots# of BasesSizePublished
SRR83567948,925,044519.1M229.8Mb2023-03-23
SRR83567957,301,342421.6M192.4Mb2023-03-23
SRR83567969,349,109546.4M235.9Mb2023-03-23
SRR83567977,854,624455.8M203.4Mb2023-03-23

ID:
6982172

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