GSM3521715: kdrl:mCherry+;sele:GFP+, 72 hpf; Danio rerio; RNA-Seq - SRA

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SRX5167559: GSM3521715: kdrl:mCherry+;sele:GFP+, 72 hpf; Danio rerio; RNA-Seq
2 ILLUMINA (Illumina HiSeq 2500) runs: 21.4M spots, 4.3G bases, 1.9Gb downloads

Submitted by: NCBI (GEO)

Study: Transcription factor induction of vascular blood stem cell niches in vivo [RNA-Seq]

PRJNA510840 • SRP173969 • All experiments • All runs

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We report gene expression data for FACS sorted zebrafish kdrl(flk1):GFP + and kdrl:mCherry+;sele(5.3kb):GFP+ double positive cells collected from whole embryos at 72 hours post fertilization (hpf). We also report gene expression data for the kdrl:GFP negative fraction. Overall design: kdrl:GFP or kdrl:mCherry;sele:GFP double positive transgenic embryos were homogenized, filtered, and sorted using FACS into PBS, collecting at least 10,000 cells for each group.

Sample: kdrl:mCherry+;sele:GFP+, 72 hpf

SAMN10617105 • SRS4175444 • All experiments • All runs

Organism: Danio rerio

Library:

Instrument: Illumina HiSeq 2500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: Total RNA was collected using Trizol LS and GenElute LPA carrier per manufacturer instructions Libraries were prepared using Ribogone kit (Clontech) and the SMARTer Universal Low input RNA Kit (Clontech; input was 50 ng total RNA for each sample) and sequenced on the Illumina Hiseq 2500

Experiment attributes:

GEO Accession: GSM3521715

Links:

NCBI link: NCBI Entrez (gds)

Runs: 2 runs, 21.4M spots, 4.3G bases, 1.9Gb

Run	# of Spots	# of Bases	Size	Published
SRR8356792	10,826,007	2.2G	1,001.4Mb	2023-03-23
SRR8356793	10,570,725	2.1G	990.6Mb	2023-03-23

ID:: 6982171

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