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SRX5638886: GSM3705591: H3K27ac_left_forebrain_EC_cell_337; Mus musculus; ChIP-Seq
1 ILLUMINA (Illumina NovaSeq 6000) run: 10,940 spots, 2.8M bases, 1.1Mb downloads

Submitted by: NCBI (GEO)
Study: CoBATCH for high-throughput single-cell epigenomic profiling [EC_CoBATCH]
PRJNA531006 • SRP190909 • All experiments • All runs
show Abstracthide Abstract
we develop a robut low input cell and high throughput single cell ChIP method used for both native and fixed in vitro cultured cells and intact tissue Overall design: we fused Protein A to the N-terminal of Tn5 to generated Protein A-Tn5 fusion enzyme, the ProteinA-Tn5-complex is enriched through specific antibodies to genomic regions and Tn5 generates indexed chromatin fragments ready for the library preparation and sequencing
Sample: H3K27ac_left_forebrain_EC_cell_337
SAMN11341304 • SRS4584351 • All experiments • All runs
Organism: Mus musculus
Library:
Instrument: Illumina NovaSeq 6000
Strategy: ChIP-Seq
Source: GENOMIC
Selection: ChIP
Layout: PAIRED
Construction protocol: Following CoBATCH protocol. After PCR enrichment using 2X KAPA master mix, products were purified and selected by AMPure XP beads for 200-1000 bp.
Experiment attributes:
GEO Accession: GSM3705591
Links:
Runs: 1 run, 10,940 spots, 2.8M bases, 1.1Mb
Run# of Spots# of BasesSizePublished
SRR885166010,9402.8M1.1Mb2019-07-16

ID:
7599942

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