Illumina HiSeq 2000 paired end sequencing; RNA-Seq of three Antarctic... - SRA

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ERX3498731: Illumina HiSeq 2000 paired end sequencing; RNA-Seq of three Antarctic invertebrates exposed to different rates of warming
1 ILLUMINA (Illumina HiSeq 2000) run: 6M spots, 1.5G bases, 495.7Mb downloads

Design: RNA-Seq of three Antarctic invertebrates exposed to different rates of warming

Submitted by: BRITISH ANTARCTIC SURVEY

Study: RNA-Seq of three Antarctic invertebrates exposed to different rates of warming

PRJEB33967 • ERP116807 • All experiments • All runs

show Abstracthide Abstract

We assembled de novo transcriptomes for three Antarctic invertebrate species: the limpet Nacella concinna (foot muscle tissue), the amphipod Paracerodocus miersii (body wall tissue) and the urchin Sterechinus neumayeri (coelomic fluid). Individuals (n = 5 per treatment) were sampled following exposure to different rates of warming: 1°C per hour, 1°C per day, 1°C per 3 days, or after acclimation to 2°C for 3 months. For longer term experiments (1°C per day, 1°C per 3 days and acclimation), control animals were sampled at both the start and end of the experiment to identify possible seasonal effects. For the shorter term experiment (1°C per hour) only one set of controls was needed.

Sample: Sn_Day_treated_5

SAMEA5861227 • ERS3649727 • All experiments • All runs

Organism: Sterechinus neumayeri

Library:

Name: Sn_Day_treated_5_p

Instrument: Illumina HiSeq 2000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: RANDOM

Layout: PAIRED

Construction protocol: Tissues were immediately sampled following the thermal treatment and snap frozen in liquid nitrogen. 1°C per 3 days end controls also act as start controls for 1°C per day due to overlap in timing of experiments.1°C per day end controls also act as end controls for 1°C per 3 days due to overlap in timing of experiments. 1°C per hour controls also act as start controls for 1°C per 3 days due to overlap in timing of experiments RNA was extracted using TRI reagent (Bioline, UK) and purified on RNeasy mini columns (Qiagen, Germany) according to manufacturer's instructions Libraries were prepared using Illumina TruSeq RNA Library Prep Kit according to manufacturer's instructions

Experiment attributes: (show all 4 attributes...) (hide...)

Experimental Factor: organism: Sterechinus neumayeri

Experimental Factor: organism part: coelomic fluid

Experimental Factor: environmental stress: 1C increase in temperature per day

Experimental Factor: cohort: Treated

Runs: 1 run, 6M spots, 1.5G bases, 495.7Mb

Run	# of Spots	# of Bases	Size	Published
ERR3477170	6,027,665	1.5G	495.7Mb	2019-09-03

ID:: 8948824

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