GSM4557340: PBS_HFD; Mus musculus; RNA-Seq - SRA

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SRX8356235: GSM4557340: PBS_HFD; Mus musculus; RNA-Seq
4 ILLUMINA (Illumina HiSeq 4000) runs: 90.6M spots, 11.5G bases, 5.7Gb downloads

Submitted by: NCBI (GEO)

Study: Diet-induced obesity promotes infection by impairment of the innate antimicrobial defense function of dermal adipocyte progenitors

PRJNA633394 • SRP262059 • All experiments • All runs

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We report the transcriptomes of single cells isolated from whole skin of wild type B6 male mice fed a standard or 60% high fat diet 48-hours after mock- or S. aureus-infection. Overall design: Wild type B6 male mice fed a standard or 60% high fat diet ad libitum were mock- or S. aureus-infected. Two days post-infection whole skin samples were collected and processed for 3'-end single cell RNA-sequencing.

Sample: PBS_HFD

SAMN14943472 • SRS6672440 • All experiments • All runs

Organism: Mus musculus

Library:

Instrument: Illumina HiSeq 4000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: Single cells were isolated as previously described (Guerrero-Juarez, et al. Nat Commun. 2019) Single cells were captured using the Chromium Platform (10X Genomics) and libraries were generated using Single Cell 3' v3 chemistry. Cell capture, library preparation, quality control, and sequencing was performed at the UCI Genomics High Throughput Facility at the University of California, Irvine. Single Cell 3' v3 chemistry - 10X Genomics

Experiment attributes:

GEO Accession: GSM4557340

Links:

NCBI link: NCBI Entrez (gds)

Runs: 4 runs, 90.6M spots, 11.5G bases, 5.7Gb

Run	# of Spots	# of Bases	Size	Published
SRR11804801	24,007,297	3G	1.5Gb	2021-05-08
SRR11804802	22,741,796	2.9G	1.4Gb	2021-05-08
SRR11804803	27,646,597	3.5G	1.8Gb	2021-05-08
SRR11804804	16,253,901	2.1G	1Gb	2021-05-08

ID:: 10869200

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