Instrument: NextSeq 500
Strategy: OTHER
Source: GENOMIC
Selection: other
Layout: PAIRED
Construction protocol: To prepare the material for 3C-seq library construction, cells were crosslinked, permeabilized, and treated with AluI. The DNA was then subjected to proximity ligation, crosslink reversal, and phenol extraction followed by isopropanol precipitation. Libraries were constructed using NEBNext Ultra DNA Library Prep Kit for Illumina.