Pleophylla navicularis targeted enrichment data - SRA

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SRX9601657: Pleophylla navicularis targeted enrichment data
1 ILLUMINA (NextSeq 500) run: 1.3M spots, 394.7M bases, 145.9Mb downloads

Design: Genomic DNA extracted using Qiagen Blood & Tissue Kits. 100 ng genomic DNA (if available) was sheared with a Bioruptor PICO sonicator to gain approximately 350 bp long DNA fragments. End-repair and A-tailing with Agilent SureSelectXT2 Reagent Kit, NEBNext Quick Ligation Module, NEBNext Multiplex Oligos for Illumina (Dual Index Set1) and NEBNext Q5 HotStart HiFi PCR Master Mix were used for pre-capture adaptor ligation and dual-indexing amplification. PCR-program: ten or twelve or 16 cycles (98C/10s65C/75s) the number of cycles was dependent on concentrations after a-tailing concentration measurement. Between these steps, samples were purified with AMPure XP beads. After amplification, equal DNA amounts of eight samples a time were pooled. SureSelect XT2 Pre-Capture ILM Module Box 2 Kit was used for the following hybridization steps. After dsDNA was denatured for 5 min at 95 C in a thermal cycler, hybridization took place at 65 C for approximately 48 h. The library of hybridized baits and DNA fragments was captured with Dynabeads MyOne Streptavidin T1 beads. The captured libraries were amplified in twelve cycles (for bad samples 14 cycles) using the SureSelect XT2 primer mix and Herculase II PCR Master Mix and purified with AMPure XP beads. Libraries were sequenced on an Illumina Nextseq 500 platform with 260 million reads.

Submitted by: Zoological Research Museum Alexander Koenig

Study: Standardized universal nuclear markers advance Metazoan taxonomy

PRJNA679846 • SRP294737 • All experiments • All runs

show Abstracthide Abstract

The goal of this project is to investigate the suitability of Universal Single Copy Orthologs (USCOs) as a set of markers useful for species-level taxonomy in all metazoans. For this, targeted enrichment of USCO loci from various animal taxa was conducted and the resulting sequences were used for phylogenetic, population genetic and species delimitation analyses.

Sample:

SAMN16856573 • SRS7805434 • All experiments • All runs

Organism: Pleophylla navicularis

Library:

Name: T147

Instrument: NextSeq 500

Strategy: Targeted-Capture

Source: GENOMIC

Selection: Hybrid Selection

Layout: PAIRED

Runs: 1 run, 1.3M spots, 394.7M bases, 145.9Mb

Run	# of Spots	# of Bases	Size	Published
SRR13162807	1,314,929	394.7M	145.9Mb	2021-12-01

ID:: 12545094

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